The aim of our present study is to evaluate the antidiabetic activity of proteases extracted from specific protein antigen, bovine serum albumin bsa using calotropis gigantea, flowers dissolved. In ovalbumin induced asthma, rats were sensitized and challenged with ovalbumin ova. Calotropis procera is a species of flowering plant in the family apocynaceae that is native to north africa, tropical africa, western asia, south asia, and indochina. Extraction of plant latex is a complex emulsion of polymer micro particles in an aqueous medium. The antimicrobial activity of crude aqueous extract of latex of calotropis gigantea apocynaceae was tested against six pathogenic species of bacteria staphylococcus aureus, bacillus cereus, escherichia coli, micrococcus luteus, klebsiella pneumoniae and pseudomonas aeruginosa and two species of fungi candida krusei and aspergillus niger. Understanding how this highly adapted plant persists in harsh environments should inform future efforts to improve the hardiness of crop and forage plant species. All structured data from the file and property namespaces is available under the creative commons cc0 license. Leaves and flowers in streptozotocininduced diabetic rats. Wound healing activity of latex of c alotropis gigantea article pdf available in international journal of pharmacy and pharmaceutical sciences 11. Calotropis gigantea grows to a height of 8 to 10 ft 2.
Invitro studies on calotropis procera leaf extracts as inhibitors of. Giganteol which was first obtained from the stem bark is one of them and a new triterpene alcohol, isogiganteol is the other. Calotropis gigantea linn apocynaceae is a glabrous or hoary, laticiferous shrubs or small trees, about 34 m tall commonly known as the swallowwort or milkweed. It has one of the important traditional medicines to treat. Cns activity of calotropis gigantea roots sciencedirect. Evaluation of antibacterial activity of calotropis gigantea latex extr. Fresh calotropis gigentica leaves were collected from the wasteland of jaipur and after washing shade dried at room temperature for 34 days.
An excellent, if rather terse, guide to the traditional medicinal uses of the plants of the region in nepal, the latex is applied to sprains, body pains, boils and pimples. Antidiabetic activity of ethanol extract of cassia klenii leaf in streptozotocininduced diabetic rats and isolation of an active fraction and toxicity evaluation of the extract. Singh n, jain nk, kannojia p, garud n, pathak ak, mehta sc, in vitro antioxidant activity of calotropis gigantea hydroalcohlic leaves extract. An recent approach on calotropis gigantea and calotropis procera. Chitme institute of pharmacy, bundelkhand university, jhansi, uttar pradesh, india ramesh chandra bundelkhand university, jhansi, uttar pradesh, india sadhna kaushik department of pharmacology, m. Pdf many drugs commonly used today are of herbal origin. Aiton is a spreading shrub or mediumsized tree reaching 2. Phytochemical analysis, antibacterial and antioxidant activity of. Calotropis gigantea crown flower is a species of calotropis native to.
Table 1 showed preliminary phytochemical screening of plant parts of calotropis gigantea. Inhibitory effect of calotropis gigantea extract on. Small trees or shrubs, these two species are native to southern asia and africa and were introduced to south america and the islands of the caribbean, where they have naturalized. Preliminary phytochemical screening of calotropis gigantea. Calotropis procera, latex, antimicrobial, antioxidant, antifungal. Evaluation of antibacterial activity of calotropis gigentica against.
Significant activity was shown by calotropis gigantea doses as compared to control. Study on antibacterial activity of calotropis procera. Each flower consists of five pointed petals and a small, elegant crown rising from the centre, which holds the stamens. This page was last edited on 15 december 2019, at 08. Pdf antidiabetic activity of calotropis gigantea in human. As an important medicinal shrub and a fiber resource plant, there is an urgent need for developing molecular markers to facilitate breeding and genetic improvement of varieties. As a continuation, the chemical constituents present in ethyl acetate extract of calotropis gigantea l. Antimicrobial activity and phytochemical analysis of. The study was approved by the institutional animal ethical committee of b. In this study, using the illumina high throughput sequencing.
Can you please tell me the place from where i can get them in large quantity so that my work can be easily started. Pdf antimicrobial properties of ocimum sanctum and. Mudarol, the chief resinol component of the root bark ofcalotropis gigantea, has been separated into two isomeric compounds by fractional crystallisation of the acetates and also by chromatographic adsorption analysis of the free alcohols. Antimicrobial activity of latex of calotropis gigantea against pathogenic. Evaluation of antibacterial activity of calotropis. Previous studies on the antimicrobial activity of c.
Studies on antidiarrheal activity of calotropis gigantea r. Studies on antidiarrhoeal activity of calotropis gigantea. The purpose of the present study was to evaluate scientifically the antidiarrhoeal effects of c. Different biochemical screening have been carried out to identify the important phyto. Evaluation of antibacterial activity of calotropis gigentica. The extractions from latex are quit tricky in context with preparation of latex for the extraction purpose. Pharmpcology, nri institute of pharmaceutical sciencesnips, bhopal 2asst professor, nips, bhopalmp krishna. The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of calotropis procera and calotropis gigantea.
Extracts on growth and survival dynamics of selected pathogenic microorganisms the growth profiles of c. Preliminary phytochemical screening and gc ms issn profiling. Antifungal, antioxidant and antibacterial activities of. Chitme hr, chandra r, kaushik s, evaluation of antipyretic activity of calotropis gigantea asclepiadaceae in experimental animals. Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology, university of science and technology bannu, khyber pakhtunkhwa pakistan key words. University rewa india nbri lucknow india abstract herbals plants are effective source of traditional and. Files are available under licenses specified on their description page. Calotropis gigantea is one such plant found widespread in most of the agricultural and nonagricultural fields. Antibacterial activity of aqueous extract of calotropis.
Antimicrobial activity of latex silver nanoparticles. Antifungal, antioxidant and antibacterial activities of calotropis procera mushtaq ahmed, rehmat ali khan, s. Calotropis procera is a well known plant and has been traditionally used for diarrhea, stomacic, sinus, fistula, and skin disease, alikhan and khanum 2005, raghubir et al. In both models the common parameter determined was ulcer index. Ethanolic extract of calotropis gigantea at a dose of 150 and 300mgkg produced significant inhibition of the gastric lesions induced by pylorus. A downloadable pdf document of a book in prepublication awaiting illustration. An in vitro study venkatesan saratha and sorimuthu pillai subramanian department of biochemistry, university of madras guindy campus, chennai, tamil nadu, india abstract medicinal plants are important source of potentially useful. Preliminary phytochemical screening of calotropis gigantea leaf. Pdf antidiabetic activity of calotropis gigantea in. Extracts on growthand survival dynamics of selected pathogenic microorganisms david m1, bharath k r2, bhavani m3 1,3department of biotechnology, sir padampat singhania university, udaipur, 3601, india. The extraction was performed for 24 h with methanol. Asclepiadaceae was tested orally in albino rats at the dose level of 250 and 500 mgkg bodyweight for cns activity. Abstractcalotropis procera and calotropis gigantea are medicinal. Calotropis gigantea is an important indian medicinal plant and widely used in ayurveda for management of various diseases.
Calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed. Present study was undertaken to investigate the effect of methanolic extract of root of calotropis gigantea linn. Antioxidant, antimicrobial activities and gcms analysis of. The hypoglycemic activity in normal rats was carried out by treatment using chloroform. Chemical composition of calotropis gigantea springerlink. Asclepiadaceae a wildly growing plant has been reported to possess number of medicinal properties and other purposes.
Studies on antidiarrhoeal activity of calotropis gigantea r. International research journal of pharmaceutical and. General information crown flower is a fastgrowing, attractive, evergreen flowering shrub or small tree that grows about 5 metres tall, occasionally to 10 metres. Preliminary phytochemical screening of calotropis gigantea leaf suchita singh, sanchita singh, r. Sep 22, 2015 the sodom apple calotropis gigantea, a member of the asclepiadaceae family, is a large evergreen shrub native to continental asia and northern africa.
It has a deep taproot, 34 m deep, and a secondary root system with woody lateral roots that may rapidly regenerate adventitious shoots when the plant is injured. Efficacy of antibacterial activity of calotropis gigantea. The agar diffusion test was used to check the antimicrobial activity of the. Preliminary phytochemical screening and gc ms issn. Calotropis gigantea and calotropis procera are to a great extent having a very similar chemical properties, but the colours of their flowers are different. Review of literature ayurvedic system of medicine is widely practised and accepted by an estimated 65% of the population in rural areas in india. The latex of calotropis gigantea contains cardio glycosides, volatile fatty acids and calcium oxalate.
Pdf antimicrobial activity of akanda calotropis gigantea l. Phytochemical investigation of different plant parts of. The leaves are broadly elliptical to oblongobovate in shape, with the size of 920 cm x 612. Batch extraction the objective of the batch experiments is to measure the global kinetics of extraction and to investigate the influences of the operating parameters. The green fruits contain a toxic milky sap that is extremely bitter and turns into a gluey coating which is resistant to soap. The extracts were filtered with whatman filter paper to obtain a clear. Inhibitory effect of calotropis gigantea extract on ovalbumin. Calotropis belongs to asclepiadaceae or milkweed or ak family which includes 280 genera and 2,000 species of worldwide distribution but most abundant in the subtropics and tropics, and rare in cold countries. The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Alcoholic extract of peeled roots of calotropis gigantea r. But the 200 mg kg dose of ethanolic extract calotropis gigantea showed maximum anti inflammatory effect and was comparable to that produced by 100mg kg of. Calotropis procera is a wild plant species in the family apocynaceae that is able to grow in harsh, arid and heat stressed conditions.
Br leaves eecg was investigated in pylorus ligation and ethanol induced ulcer models in experimental rats. The 11 analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and 12 flower of calotropis procera was carried out in disc method and also determined mic value at 600nm through optical density using spectrophotometer. Provided by smithsonian institution, department of botany. International journal of scientific and research publications, volume 4, issue 2, february 2014 1 issn 22503153. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. Antibacterial and phytochemical studies on calotropis gigantia l. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli. Dec 03, 2010 to evaluate the hypoglycemic and antidiabetic activity of chloroform extract of calotropis gigantea leaves and flowers in normal rats and streptozotocin induced diabetes. The latex of the plant is also reported to possess analgesic and wound healing activity 19, 20, as well as antiinflammatory 21 and antimicrobial activities 22.
The ph of latex of these two species has been found different in the present study. The leaves are broadly elliptical to oblongobovate in shape, with the size of 9. It is widely used in many ayurvedic treatments, both externally ksharasutra and internally. Transcriptomic and metabolic responses of calotropis. The nematodes meloidogyne incognita and meloidogyne javanica are found on the roots of calotropis gigantea in india, although the leaf extract kills them. Calotropis gigantea is a well known medicinal herb commonly known as madar has been used in unani, ayurveda, and siddha system of medicine for years.
Other articles where calotropis gigantea is discussed. Venkatesan saratha and sorimuthu pillai subramanian department of biochemistry, university of madras guindy campus, chennai, tamil nadu, india. Studies on antidiarrheal activity of calotropis gigantea. Ramesh chandra bundelkhand university, jhansi, uttar pradesh, india. Prominent analgesic activity was observed in eddys hot plate method and acetic acid induced writhings. Pdf the antibacterial activity of methanol extract from the root bark of akanda calotropis gigantea l. The first species being abundant while the second being restricted to forest areas. The leaves are sessile and subsessile, opposite, ovate, cordate at the base. The chloroform extracted fraction of latex showed inhibitory effect against s. Calotropis gigantea latex, antifungal activity, minimum fungicidal concentration, zone of inhibition, disc diffusion method correspondence to author.
The antibacterial activity of methanol extract from the root bark of akanda calotropis gigantea l. Pdf antimicrobial activity of latex of calotropis gigantea against. Calotropis species recorded for a number of activities like. The results of anti inflammatory effect of calotropis gigantea are showed in table 2. Three hundred grams of powdered calotropis was separately macerated with 100% ethanol. The antiulcer activity of ethanolic extract of calotropis gigantea r. Pdf wound healing activity of latex of calotropis gigantea.
The plant is erect, tall, large, much branched and perennial with milky latex throughout. Chemical characterization and insecticidal activity of. Chitme 1 institute of pharmacy, bundelkhand university, jhansi, uttar pradesh, india. Pdf the antimicrobial activity of crude aqueous extract of latex of calotropis gigantea apocynaceae was tested against six pathogenic species of. To evaluate antimicrobial and phytochemical activity of hexane and ethanolic extract of.
Studies on endophytic fungi of calotropis procera l. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria. It is a body reaction in order to eliminate or limit the spread of injurious agent as. The plant has oval, light green leaves and milky stem. It is a very common herb seen widely throughout india. The ayurveda medicine system and medicinal plants help to meet their primary health care needs farnsworth et al. The paw licking time was delayed and the numbers of writhings were greatly reduced.
Name of species calotropis gigantea common name milkweed, swet ark family apocynaceae photo taken on 12 july, 2008 at sadashiv jyotirling, kukas, jaipur, rajasthan description dry leaf powder used for treating wounds and boils. Result of the antimicrobial activity of root extracts of calotropis gigantea. Evaluation of antibacterial activity of calotropis gigentica against streptococcus mutans and lactobacillus acidophilus. Asclepiadaceae, commonly known as milkweed or swallowwort, is a common wasteland weed singh et al. Introduction inflammation is defined as the local response of living mammalian to injury due to agent. Both of methanol extract and its chloroform fraction showed activity against sarcina lutea, bacillus megaterium and pseudomonas aeruginosa. The sodom apple calotropis gigantea, a member of the asclepiadaceae family, is a large evergreen shrub native to continental asia and northern africa. Plants profile for calotropis gigantea giant milkweed. Antimicrobial activity of akanda calotropis gigantea l. I am a research scholar and want to collect flowers of calotropis gigantea flowers for my study. Antimicrobial activities of calotropis procera on selected. The search for novel antibiotics like compounds from medicinal plants. Ocimum sanctum and calotropis gigantea leaves on clinical pathogens.
Sadhna kaushik department of pharmacology, maharani laxmi bai medical college, jhansi, uttar pradesh, india. Calotropis gigantea is a common wasteland weed and known for various medicinal properties. University rewa india nbri lucknow india abstract herbals plants are effective source of traditional and modern medicines, useful for primary health care. Experimental and modeling studies on extraction of amyrins. Cg on ovalbumin induced asthma and arachidonic acid induced paw edema in rats.