The basic local alignment search tool blast finds regions of local similarity between sequences. Blast command line applications user manual ncbi bookshelf. This manual documents the blast basic local alignment search tool command line applications developed at the national center for biotechnology. In order to help users arrive at an answer to this. A glossary of terms you will see when using the tool. Indepth information on the tools functionality and features. Appendices blast command line applications user manual. Blast basic local alignment search tool blast program selection guide table of content 1. Ncbi has provided blast sequence analysis services for over a decade. Blast2go user manual blast2go file creation for ncbi submission of complete eukaryotic genomes or chromosomes may, 2016 biobam bioinformatics s. Blast can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families. Download blast software and databases documentation.
This allows users to perform blast searches on their own server without size, volume and database restrictions. The ncbi manual covers quite a few powerful and handy features of blast on the. This process is repeated as many times as the user wishes, with more. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. A blast search against a database requires at least a query and db option. Blast command line applications user manual internet. Command line blast a primer for computational biology. Videos from the national center for biotechnology information including presentations and tutorials about ncbi biomolecular and biomedical literature databas. A service of the national library of medicine, national institutes of health.